Laparoscopic Gastroileal Bypass with Single Anastomosis: Analysis of the First 1512 Patients.

BACKGROUND: Biliopancreatic diversion is perceived as the most effective operation for long-term treatment of massive obesity. The purpose of this study is to demonstrate that gastroileal bypass with single anastomosis is a safe and feasible procedure with similar results to the classic derivation, but reducing surgical time without decreasing the efficacy. METHODS: Descriptive, observational, prospective study of patients undergoing gastroileal bypass with single anastomosis between April 2010 and December 2015. The postoperative follow-up was 24 months. RESULTS: One thousand five hundred twelve patients underwent gastroileal bypass. The mean time of the procedure was 32 min; the average stay was 2.2 days. 30.1% of patients lost more than 100% of their excess weight, and 72.35% of patients lost more than 75% of their excess weight. 95.17% of patients dropped to a BMI < 35; 75.99% to a BMI < 30 and 30.15% to a BMI < 25. CONCLUSIONS: Gastroileal bypass with single anastomosis is a safe and fast procedure providing similar results to biliopancreatic diversion with respect to weight loss.

Knockdown of PTRF ameliorates adipocyte differentiation and functionality of human mesenchymal stem cells.

Healthy expansion of human adipose tissue requires mesenchymal stem cells (hMSC) able to proliferate and differentiate into mature adipocytes. Hence, characterization of those factors that coordinate hMSC-to-adipocyte transition is of paramount importance to modulate the adipose tissue expansion. It has been previously reported that the adipogenic program of hMSC can be disrupted by upregulating caveolar proteins, and polymerase I and transcript release factor (PTRF) is an integral component of caveolae, highly expressed in adipose tissue. Here, we hypothesized that the role of PTRF in adipocyte functionality might stem from an effect on hMSC. To test this hypothesis, we isolated hMSC from the subcutaneous fat depot. We found an upregulated expression of the PTRF associated with decreased adipogenic potential of hMSC, likely due to the existence of senescent adipocyte precursors. Employing short hairpin RNA-based constructs to stably reduce PTRF, we were able to restore insulin sensitivity and reduced basal lipolysis and leptin levels in human adipocytes with high levels of PTRF. Additionally, we pinpointed the detrimental effect caused by PTRF on the adipose tissue to the existence of senescent adipocyte precursors unable to proliferate and differentiate into adipocytes. This study provides evidence that impaired adipocyte functionality can be corrected, at least partially, by PTRF downregulation and warrants further in vivo research in patients with dysfunctional adipose tissue to prevent metabolic complications.